Description of the human hprt database and software

Database

The most recent hprt database contains information on over 2,300 mutations found in vivo and in vitro in the human hprt gene and runs under Windows. The version for evaluation on this homepage has fewer mutations and is a DOS program.

The database contains information on the mutagen, dose, spontaneous and induced mutant fraction, base position, amino acid position, amino acid change, local DNA sequence, cell type, citation, and other items. In addition, information regarding the cause and effect of mutations affecting splicing is given.

The database itself is in the dBASE format.

Software

Routines have been developed for the analysis of single base substitutions, including programs to:

  • (i) determine if two mutational spectra are different
  • (ii) determine if mutations show a DNA strand bias
  • (iii) determine the frequency of transitions and transversions
  • (iv) examine the number of mutations and mutable sites in each exon
  • (v) display the number and kind of mutations observed at each base in the coding region
  • (vi) perform nearest neighbor analysis
  • (vii) display mutable amino acids in the hprt protein.

    • The software runs only on IBM-compatible PCs.

    Availablity

    The latest version of the database and software is now available on a subscription basis. However, an older version is present at this site for evaluation. The new version of the software runs under Microsoft Windows.

    For more information about the new version please send EMAIL note to:

    Neal Cariello (cariello@sunsite.unc.edu)

    You can download an older version of the hprt database and software for evaluation.

    References

    1. Cariello NF et al. Human hprt mutant database: software for data entry and retrieval. Environ. Molec. Mutagen., 20, 81-83, 1992.
    2. Cariello NF and Skopek TR. Analysis of mutations occurring at the human hprt locus. J. Mol. Biol., 231, 41-57, 1993.
    3. Cariello NF and Skopek TR. In vivo mutations at the human hprt locus. Trends in Genetics, 9, 322-326, 1993.
    4. Cariello NF. Software for the analysis of mutations at the human hprt locus. Mutation Res., 312, 173-185, 1994.
    5. Cariello NF. Database and software for the analysis of mutations at the human hprt gene. Nucl. Acids Res., 22, 3547-3548, 1994.
    6. Cariello NF, Douglas GR and Soussi T. Databases and software fo the analysis of mutations in the human p53 gene, the human hprt gene and the lacZ gene in transgenic rodents. Nucl. Acids Res., 24, 119-120, 1996.