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Re: [nafex] Sterilizing scions

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Yes, ELISA (Enzyme-Linked ImmunoSorbent Assay) is still a major way to detect viruses in plant tissue.  PCR 
is becoming more common, but ELISA based on polyclonal antiserum will generally pick up more variants of 
the same virus, whereas PCR may miss specific variants.  Either test can be done in a day, but ELISAs are 
often done in two days for convenience and increased sensitivity.  

Yorrba

On Sat, 24 Feb 2001 18:20:49 -0800, Lon J. Rombough wrote:

>Use of indicator plants is the older method.  More commonly, serum tests are
>used now that give results in a few days in most cases.  Joseph Postman -
>can you give us more info?  Is the ELIZA test still the main one? 
>----------
>>From: jhecksel@voyager.net
>>To: nafex@yahoogroups.com
>>Subject: Re: [nafex] Sterilizing scions
>>Date: Sat, Feb 24, 2001, 9:14 PM
>>
>
>>This thread is similar to one we had on Prunus seeds.  If I remember
>>write, the major concern is virus (virii?).  Chlorine bleach will do a
>>fine job sterilizing the exterior of the scion, but virus is in the
>>cells.
>>
>>Cultivars are cleaned up by budding onto a very vigorous rootstock and
>>then providing optimum growth conditions...maybe just a bit on the warm
>>side.  The warmth slows down the virus infection and the growing tip of
>>the plant out runs the virus...if only by a little bit.
>>
>>The very end of the whip is harvested while it is still growing at a
>>gang-buster rate.  It is grafted onto a virus free rootstock and housed
>>in a chamber.  Buds are removed from the plant that is now *hopefully*
>>virus free and are placed on indicator plants.  Indicator plants are
>>cultivars that produce visible symptoms when infected with virus
>>diseases.  This is a key point:  With most cultivars/species, you cannot
>>tell if a plant has virus.  These plants without visible symptoms are
>>called "latent".  Moving buds to indicator plants is done several times
>>jsut to be sure.  If all the indicator plants are symptom free after a
>>waiting period, the plant is considered free of latent virus.
>>
>>This process is a little more involved than most home fruit growers are
>>willing to go through.  You need several insect proof enclosures as well
>>as vigorous rootstocks and indicator plants.  But I believe that USDA is
>>willing to licence you for importation if you can demonstrate you can
>>and will follow the correct protocols.
>>
>
>
> 
>
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>
>
>

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