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[SANET-MG] DNA in food and feed
http://www.i-sis.org.uk/GMDNAIF.phpISIS Press Release 17/06/04
DNA in GM Food & Feed
The government’s scientific advisory committees have repeatedly tried to
reassure the public that there is nothing to fear from genetically
modified (GM) DNA, but critics disagree. Dr. Mae-Wan Ho offers a quick
guide for the perplexed
A fully referenced version of this article is posted on ISIS Members’
website. Details here.
Is GM DNA different from natural DNA?
"DNA is DNA is DNA," said a proponent in a public debate in trying to
convince the audience that there is no difference between genetically
modified (GM) DNA and natural DNA, "DNA is taken up by cells because it
is very nutritious!"
"GM can happen in nature," said another proponent. "Mother Nature got
there first."
So, why worry about GM contamination? Why bother setting contamination
thresholds for food and feed? Why award patents for the GM DNA on
grounds that it is an innovation? Why don’t biotech companies accept
liabilities if there’s nothing to worry about?
As for GM happening in nature, so does death, but that doesn’t justify
murder. Radioactive decay happens in nature too, but concentrated and
speeded up, it becomes an atom bomb.
GMDNA and natural DNA are indistinguishable according to the most
mundane chemistry, i.e., they have the same chemical formula or atomic
composition. Apart from that, they are as different as night and day.
Natural DNA is made in living organisms; GMDNA is made in the
laboratory. Natural DNA has the signature of the species to which it
belongs; GMDNA contains bits copied from the DNA of a wide variety of
organisms, or simply synthesized in the laboratory. Natural DNA has
billions of years of evolution behind it; GMDNA contains genetic
material and combinations of genetic material that have never existed.
Furthermore, GMDNA is designed – albeit crudely - to cross species
barriers and to jump into genomes. Design features include changes in
the genetic code and special ends that enhance recombination, i.e.,
breaking into genomes and rejoining. GMDNA often contains antibiotic
resistance marker genes needed in the process of making GM organisms,
but serves no useful function in the GM organism.
The GM process clearly isn’t what nature does (see "Puncturing the GM
myths", SiS22). It bypasses reproduction, short circuits and greatly
accelerates evolution. Natural evolution created new combinations of
genetic material at a predominantly slow and steady pace over billions
of years. There is a natural limit, not only to the rate but also to the
scope of gene shuffling in evolution. That’s because each species comes
onto the evolutionary stage in its own space and time, and only those
species that overlap in space and time could ever exchange genes at all
in nature. With GM, however, there’s no limit whatsoever: even DNA from
organisms buried and extinct for hundreds of thousands of years could be
dug up, copied and recombined with DNA from organisms that exist today.
GM greatly increases the scope and speed of horizontal gene transfer
Horizontal gene transfer happens when foreign genetic material jumps
into genomes, creating new combinations (recombination) of genes, or new
genomes. Horizontal gene transfer and recombination go hand in hand. In
nature, that’s how, once in a while, new viruses and bacteria that cause
disease epidemics are generated, and how antibiotic and drug resistance
spread to the disease agents, making infections much more difficult to
treat.
Genetic modification is essentially horizontal gene transfer and
recombination, speeded up enormously, and totally unlimited in the
source of genetic material recombined to make the GMDNA that’s inserted
into the genomes plants, animals and livestock to create genetically
modified organisms (GMOs).
By enhancing both the rate and scope of horizontal gene transfer and
recombination, GM has also increased the chance of generating new
disease-causing viruses and bacteria. (It is like increasing the odds of
getting the right combination of numbers to win a lottery by betting on
many different combinations at the same time.) That’s not all. Studies
on the GM process have shown that the foreign gene inserts invariably
damages the genome, scrambling and rearranging DNA sequences, resulting
in inappropriate gene expression that can trigger cancer.
The problem with the GM inserts is that they could transfer again into
other genomes with all the attendant risks mentioned. There are reasons
to believe GM inserts are more likely to undergo horizontal transfer and
recombination than natural DNA, chief among which is that the GM inserts
(and the GM varieties resulting from them) are structurally unstable,
and often contain recombination hotspots (such as the borders of the
inserts).
After years of denial, some European countries began to carry out
‘event-specific’ molecular analyses of the GM inserts in commercially
approved GM varieties as required by the new European directives for
deliberate release, novel foods and traceability and labelling. These
analyses reveal that practically all the GM inserts have fragmented and
rearranged since characterised by the company. This makes all the GM
varieties already commercialised illegal under the new regime, and also
invalidates any safety assessment that has been done on them (see
"Transgenic lines proven unstable", SiS 20 and "Unstable transgenic
lines illegal", SiS 21). As everyone knows, the properties of the GM
variety, and hence its identity, depend absolutely on the precise form
and position of the GM insert(s). There is no sense in which a GM
variety is "substantially equivalent" to non-GM varieties.
GMDNA in food & feed
In view of the strict environmental safety assessment required for
growing GM crops in Europe, biotech companies are bypassing that by
applying to import GM produce for food and processing only. Is GM food
safe? There are both scientific and anecdotal evidence indicating it may
not be: many species of animals were adversely affected after being fed
different species of GM plants with a variety of GM inserts (see "GM
food safe?" series, SiS 21), suggesting that the common hazard may
reside in the GM process itself, or the GMDNA.
How reliably can GMDNA be detected?
DNA can readily be isolated and quantified in bulk. But the method
routinely used for detecting small or trace amounts of GMDNA is the
polymerase chain reaction (PCR). This copies and amplifies a specific
DNA sequence based on short ‘primers strings’ of DNA that match the two
ends of the sequence to be amplified, and can therefore bind to the ends
to ‘prime’ the replication of the sequence through typically 30 or more
cycles, until it can be identified after staining with a fluorescent dye.
There are many technical difficulties associated with PCR amplification.
Because of the small amount of the sample routinely used for analysis,
it may not be representative of the sample, especially if the sample is
inhomogeneous, such as the intestinal contents of a large animal. The
primers may fail to hybridise to the correct sequence; the PCR itself
may fail because inhibitors are present. Usually, the sequence amplified
is a small fraction of the length of the entire GM insert, and will
therefore not detect any other GM fragment present. If the target
sequence itself is fragmented or rearranged, the PCR will also fail. For
all those reasons, PCR will almost always underestimate the amount of
GMDNA present, and a negative finding cannot be taken as evidence that
GMDNA is absent.
A new review on monitoring GM food casts considerable doubt over the
reliability of PCR methods. Mistakes can arise if the sample is not
large enough to give a reliable measure, or if the batch of grain
sampled is inhomogeneous, or the PCR reaction not sensitive enough, or
the data presented to the regulatory authorities simply not good enough.
Consequently, the level of contamination is almost invariably
underestimated.
There is an urgent need to develop sensitive, standardized and validated
quantitative PCR techniques to study the fate of GMDNA in food and feed.
Regulatory authorities in Europe are already developing such techniques
for determining GM contamination. One such technique has brought the
limit of detection down to 10 copies of the transgene (the GM insert or
a specific fragment of it).
In contrast, the limit of PCR detection in investigations on the fate of
GMDNA in food and feed is extremely variable. In one study commissioned
by the UK Food Standards Agency, the limit of detection varied over a
thousand fold between samples, with some samples requiring more than 40
000 copies of the GM insert before a positive signal is registered. Such
studies are highly misleading if taken at face value, given all the
other limitations of the PCR technique.
Despite that, however, we already have answers to a number of key
questions regarding the fate of DNA in food and feed.
1. Does DNA break down sufficiently during food processing?
The answer is no, not for most commercial processing. DNA was found to
survive intact through grinding, milling or dry heating, and
incompletely degraded in silage. High temperatures (above 95 deg. C) or
steam under pressure were required to degrade the DNA completely.
"The results imply that stringent conditions are needed in the
processing of GM plant tissues for feedstuffs to eliminate the
possibility of transmission of transgenes." The researchers warned.
They pointed out for example, that the gene aad, conferring resistance
to the antibiotics streptomycin and spectinomycin, is present in GM
cottonseed approved for growth in US and elsewhere (Monsanto’s Bollgard
(insect-protected) and Roundup Ready (herbicide tolerant)). Streptomycin
is mainly used as a second-line drug for tuberculosis. But it is in the
treatment of gonorrhoea that spectinomycin is most important. It is the
drug of choice for treating strains of Neisseria gonorrhoeae already
resistant to penicillin and third generation cephalosporins, especially
during pregnancy. The release of GM crops with the blaTEM gene for
ampicillin resistance is also relevant here, because that’s where
resistance to cephalosporins has evolved.
Another study found large DNA fragments in raw soymilk of about 2 000bp
(base pairs, unit of measurement for the length of DNA), which degraded
somewhat after boiling, but large fragments were still present in tofu
and highly processed soy protein. Heating in water under acid conditions
was more effective in degrading DNA, but again, the breakdown was
incomplete (fragments larger than 900bp remaining).
It is generally assumed, incorrectly, that DNA fragments less than 200bp
pose no risk, because they are well below the size of genes. But that’s
a mistake, as these fragments may be promoters (signals needed by genes
to become expressed), and sequences of less than 10bp can be binding
sites for proteins that boost transcription. The CaMV 35S promoter, for
example, is known to contain a recombination hotspot, and is implicated
in the instability of GM inserts.
2. Does DNA break down sufficiently rapidly in the gastrointestinal tract?
Although free DNA breaks down rapidly in the mouth of sheep and humans,
it was not sufficiently rapid to prevent gene-transfer to bacteria
inhabiting the mouth. DNA in GM food and feed will survive far longer.
The researchers conclude: "DNA released from feed material within the
mouth has potential to transform naturally competent oral bacteria."
Several studies have now documented the survival of DNA in food
throughout the gastrointestinal tract in pig and mice, in the rumen of
sheep and in the rumen and duodenum of cattle. The studies were variable
in quality, depending especially on the sensitivity of the PCR
methodology used to amplify specific sequences for detection.
Nevertheless they suggest that GMDNA can transfer to bacteria within the
rumen and in the small intestine. In neither sheep nor cattle was feed
DNA detected in the faeces, suggesting that DNA breakdown may be
complete by then.
The only feeding trial in human volunteers was perhaps the most
informative. After a single meal containing GM soya containing some
3x1012 copies of the soya genome, the complete 2 266 bp epsps transgene
was recovered from the colostomy bag in six out of seven ileostomy
subjects (who had their lower bowel surgically removed). The levels were
highly variable among individuals as quantified by a small 180bp PCR
product overlapping the end of cauliflower mosaic virus (CaMV) 35S
promoter and the beginning of the gene: ranging from 1011 copies (3.7%)
in one subject to only 105 copies in another. This is a strong
indication that DNA in food is not sufficiently rapidly broken down in
transit through the gastrointestinal tract, confirming the results of an
earlier experiment by the same research group.
No GMDNA was found in the faeces of any of 12 healthy volunteers tested,
suggesting that DNA has completely broken down, or all detectable
fragments have passed into the bloodstream (see later) by the time food
has passed through the body. This finding is in agreement with the
results from ruminants.
In general, the studies report that GMDNA degrades to about the same
extent and at about the same rate as natural plant DNA. However, no
quantitative measurements have been made, and GMDNA was often compared
with the much more abundant chloroplast DNA, which outnumbers the
transgene by 10 000 to one.
3. Does GMDNA get taken up by bacteria and other micro-organisms?
The answer is yes. The evidence was reported in the human feeding trial
mentioned. The transgene was not detected in the content of the
colostomy bag from any subject before the GM meal. But after culturing
the bacteria, low levels were detected in three subjects out of seven:
calculated to be between 1 and 3 copies of the transgene per million
bacteria.
According to the researchers, the three subjects already had the
transgene transferred from GM soya before the feeding trial, probably by
having eaten GM soya products unknowingly. No further transfer of GM DNA
was detected from the single meal taken in the trial.
The researchers were unable to isolate the specific strain(s) of
bacteria that had taken up the transgene, which was not surprising, as
"molecular evidence indicates that 90% of microorganisms in the
intestinal microflora remain uncultured. …they can only grow in mixed
culture, a phenomenon seen with other microorganisms."
Actually, GMDNA can already transfer to bacteria during food processing
and storage. A plasmid was able to transform Escherichia coli in all 12
foods tested under conditions commonly found in processing and storage,
with frequencies depending on the food and on temperature. Surprisingly,
E. coli became transformed at temperatures below 5 degrees C, i.e. under
conditions of storage of perishable foods. In soy drink this condition
resulted in frequencies higher than those at 37 degrees C.
4. Do cells lining the gastrointestinal tract take up DNA?
The answer is yes. Food material can reach lymphocytes (certain white
blood cells) entering the intestinal wall directly, through Peyer’s
patches. And fragments of plant DNA were indeed detected in cows’
peripheral blood lymphocytes.
It is notable that in the human feeding trial, a human colon carcinoma
cell line CaCo2 was directly transformed at a high frequency of 1 in 3
000 cells by an antibiotic resistance marker gene in a plasmid. This
shows how readily mammalian cells can take up foreign DNA, as we have
pointed out some years ago (see also below).
5. Does DNA pass through the gastrointestinal tract into the blood stream?
The answer is yes, as mentioned above, fragments of plant DNA was
detected in cow’s peripheral blood lymphocytes. However, attempts to
amplify plant DNA fragments from blood have failed, most likely on
account of the presence of inhibitors of the PCR amplification.
6. Does DNA get taken up by tissue cells?
The answer is yes, and this has been known since the mid 1990s. GMDNA
and viral DNA fed to mice ended up in cells of several tissues, and when
fed to pregnant mice, the DNA was able to cross the placenta, and enter
the cells of the foetus and the newborn. These results were confirmed in
2001, when soya DNA, too, was found taken into the tissue cells of a few
animals.
In general, abundant chloroplast sequences have been detected in the
tissues of pig and chicken but not single gene DNA nor GMDNA. But rare
events are most likely to go undetected, on account of the limitations
of the PCR technique.
Recently, "spontaneous transgenesis" – the process of spontaneous uptake
of foreign DNA resulting in gene expression - has been rediscovered by a
team of researchers looking for new possibilities in gene therapy. They
documented the phenomenon in several human B lymphocyte cell lines as
well as peripheral blood B lymphocytes. The transgene in a plasmid was
readily taken up and was found in many cell compartments including the
nucleus, where gene transcription took place. The plasmid was not
integrated into the genome, but the researchers say that its eventual
integration cannot be ruled out.
7. Is GM DNA more likely to insert into genomes?
This is perhaps the most important question. There are reasons to
believe GMDNA is more likely to insert into genomes after it is taken up
into cells, chief among which, its sequence similarities (homologies) to
a wide variety of genomes, especially those of viruses and bacteria.
Such homologies are known to enhance horizontal gene transfer to
bacteria up to a billion fold.
More significantly, the integration of non-homologous genetic material
can occur at high frequencies when flanked by homologous sequences. A
recent report highlights the importance of this "homology-facilitated
illegitimate recombination", which increases the integration of foreign
(non-homologou) DNA at least 105 fold when it was flanked on one side by
a piece of DNA homologous to the recipient genome.
No experiment has yet been done to assess whether GMDNA is more likely
to transfer horizontally than natural DNA. However, in the human feeding
trial, where three ileostomy volunteers tested positive for the soya
transgene in the bacteria cultured from their colostomy bag, the soya
lectin gene Le was not detected in the bacterial cultures from any of
the subjects.
The researchers found it necessary to remark, "Although the plant lectin
gene was not detected in the microbial population…it is premature to
conclude that the epsps transgene is more likely than endogenous plant
genes to transfer into the microbial population."
But until this possibility has been adequately addressed, it cannot be
ruled out.
The Institute of Science in Society, PO Box 32097, London NW1 OXR
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