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[SANET-MG] Godzilla rice



The article below is about rice with human cytochrome p450 genes geared
for food production and pollution remediation. The carcinogenic
byproducts of the enzymes are not discussed. It looks very much like the
humanized rice will be resistant to all known herbicides. When it
escapes it will undoubtedly take over Japan. It, maybe, should be called
Godzilla rice!
Plant Science
doi:10.1016/j.plantsci.2004.10.014
Enhanced herbicide cross-tolerance in transgenic rice plants
co-expressing human CYP1A1, CYP2B6, and CYP2C19

Hiroyuki Kawahigashia, , , Sakiko Hirosea, Hideyuki Inuib, Hideo Ohkawac
and Yasunobu Ohkawaa

Abstract
We introduced an expression plasmid, pIKBACH, co-expressing the human
cytochrome P450 genes CYP1A1, CYP2B6, and CYP2C19, into rice plants
(Oryza sativa cv. ‘Nipponbare’). The transgenic rice plants (pIKBACH
rice) were screened by a combination of hygromycin resistance, PCR, and
Western blot analysis. The pIKBACH rice plants expressed all three P450
species and exhibited tolerance towards various herbicides with
different chemical structures and different modes of action, including a
photosynthesis inhibitor (chlortoluron), very long-chain fatty acids
(VLCFAs) inhibitors (acetochlor, metolachlor, and thenylchlor), a
carotenoid biosynthesis inhibitor (norflurazon), and a root-elongation
inhibitor (pyributicarb). In addition, the pIKBACH rice plants showed
high tolerance to two mixtures of three herbicides [quizalofop-ethyl
(0.15 μM), metolachlor (2 μM), and norflurazon (0.4 μM); and mefenacet
(2.5 μM), thenylchlor (2 μM), and pyributicarb (1.5 μM)]. Thin-layer
chromatography analysis revealed that the pIKBACH transgenic rice plants
exhibited high metabolic activity towards chlortoluron, metolachlor, and
norflurazon. The metabolism of herbicides by the pIKBACH rice plants was
enhanced additively by the introduced P450 species. Assuming that public
and commercial acceptance is forthcoming, pIKBACH rice plants may become
useful tools for the breeding of herbicide-tolerant crops and for
phytoremediation of environmental pollution by organic chemicals

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