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[SANET-MG] two important new papers
The first of these two papers deals with further evidence that soy food
leaves gene fragments in the organs of animals including mammals and
fish. The second article shows that transgenic DNA in soy is often
scrambled leading to read through producing novel products of yet
undetermined toxicity to those eating the soy. The DNA fragments may
also produce untoward consequences such as read through in the tissues
of those eating GM soy.
European Food Research and Technology
DOI: 10.1007/s00217-005-1160-1
Issue: Volume 221, Date: July 2005
Pages: 1 - 8
Dietary DNA in blood and organs of Atlantic salmon (Salmo salar L.)
Christer Røss Nielsen1, Knut G. Berdal1, Anne Marie Bakke-McKellep2 and
Arne Holst-Jensen1
Abstract The objective of this study was to investigate the uptake of
dietary DNA into blood, kidney, and liver of salmon, and to determine
the DNA fragment size if dietary DNA was detected. Salmon in groups of
five fish were force-fed a feed containing a high copy number of three
polymerase chain reaction (PCR) amplified DNA fragments. Tissue samples
were dissected from the fish at time intervals starting at 1 h after
force-feeding (AFF) and ending at 64 h AFF. Real-time PCR analyses were
used to determine the presence or absence of DNA targets. Sensitive
methods amplifying small fragments were used to minimise the impact of
fragmentation on the detectability of DNA targets. Uptake of dietary DNA
was observed and the highest concentrations of dietary DNA in liver and
kidney were found 8 h AFF. The results correspond to data published for
similar trials performed on other animal species. An additional
experiment showed that decontamination of the liver surface by flaming
has the potential to decrease DNA contamination from, for example, feed
remnants by up to 90%.
European Food Research and Technology
DOI: 10.1007/s00217-004-1064-5
Issue: Volume 220,
Date: March 2005 Pages: 438 - 443
Detection of RNA variants transcribed from the transgene in Roundup
Ready soybean
Andreas Rang1, , Bettina Linke and Bärbel Jansen
Abstract The acreage for genetically modified crops (GMOs)—particularly
soybean—has steadily increased since 1996, when the first crop of
Roundup Ready soybean (intended for food production) was grown. The
Roundup Ready soybean varieties derive from a soybean line into which a
glyphosate-resistant enolpyruvylshikimate-3-phosphate-synthase (EPSPS)
gene was introduced. The inserted and the flanking regions in Roundup
Ready soybean have recently been characterized. It was shown that a
further 250-bp fragment of the epsps gene is localized downstream of the
introduced nos terminator of transcription, derived from the nopaline
synthase gene from Agrobacterium tumefaciens. We examined whether this
250-bp fragment could be of functional importance. Our data demonstrate
that at least 150 bp of this DNA region are transcribed in Roundup Ready
soybean. Transcription of the fragment depends on whether read-through
events ignore the nos terminator signal located upstream. Our data also
indicate that the read-through product is further processed, resulting
in four different RNA variants from which the transcribed region of the
nos terminator is completely deleted. Deletion results in the generation
of open reading frames which might code for (as yet unknown) EPSPS
fusion proteins. The nos terminator is used as a regulatory element in
several other GMOs used for food production. This implies that read
through products and transcription of RNA variants might be a common
feature in these GMOs.
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