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[SANET-MG] production of monoclonal antibodies in crops
The paper below opens new vistas in polluting food crops. Monoclonal
antibodies are known to be highly toxic and should only be used in
clinical (not food production) environments. Here , two viruses TMV and
potato virus X are modified to produce complete antibodies for therapy.
Modified crops can nearly instantly produce quantities of the specific
antibodies to deal with pandemic or terrorism events. Of course, the
most terrifying scenario, pollution of potato crops in not discussed. Of
course, Bayer would never dream of polluting food crops with transgenes :'(
Published online before print September 14, 2006, 10.1073/pnas.0606631103
PNAS | October 3, 2006 | vol. 103 | no. 40 | 14701-14706
Anatoli Giritch*, Sylvestre Marillonnet*, Carola Engler*, Gerben van
Eldik{dagger}, Johan Botterman{dagger}, Victor Klimyuk*, and Yuri
Gleba*,{ddagger}
*Icon Genetics GmbH, Weinbergweg 22, 06120 Halle, Germany; and
{dagger}Bayer BioScience N.V., Technologiepark 38, B-9052 Gent, Belgium
Plant viral vectors allow expression of heterologous proteins at high
yields, but so far, they have been unable to express heterooligomeric
proteins efficiently. We describe here a rapid and indefinitely scalable
process for high-level expression of functional full-size mAbs of the
IgG class in plants. The process relies on synchronous co-infection and
co-replication of two viral vectors, each expressing a separate antibody
chain. The two vectors are derived from two different plant viruses that
were found to be noncompeting. Unlike vectors derived from the same
virus, noncompeting vectors effectively coexpress the heavy and light
chains in the same cell throughout the plant body, resulting in yields
of up to 0.5 g of assembled mAbs per kg of fresh-leaf biomass. This
technology allows production of gram quantities of mAbs for research
purposes in just several days, and the same protocol can be used on an
industrial scale in situations requiring rapid response, such as
pandemic or terrorism events.
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