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Dissertation Information for Shelley N. Jackson

NAME:
- Shelley N. Jackson

DEGREE:
- Ph.D.

DISCIPLINE:
- Chemistry

SCHOOL:
- Emory University (USA) (2004)

ADVISORS:
- Kermit K. Murray

COMMITTEE MEMBERS:
- Barry P. Ryan
- Vincent P. Conticello
- Joseph B. Justice Jr.

MPACT Status: Fully Complete

Title: Laser desorption mass spectrometry of bioaerosols

Abstract: Bioaerosols are airborne particles that contain biologically active compounds such as bacteria, viruses, fungal spores, and pollen and are a public health concern because they can cause infection, allergic, or toxic reaction. Traditional bioaerosol analysis methods involve particle collection and later laboratory analysis for culturing, enumeration, and characterization. In this work, the development of laser desorption mass spectrometry for the rapid analysis of bioaerosols has been explored. Analysis of bioaerosols by laser desorption mass spectrometry was conducted using both off-line and on-line techniques. In theses experiments, test bioaerosols were created using a Collison nebulizer or a fluidized bed aerosol generator. Off-line analysis of deposited bioaerosols was tested using three methods. In the first method, aerosols were impacted onto a precoated matrix target prior to analysis in a MALDI-TOF mass spectrometer. Mass peaks up to 10,000 Daltons were recorded for deposited bioaerosols using this method. In the second method, bioaerosols were coated with matrix on-line by condensation prior to deposition on a MALDI target. The coated particles were then analyzed without any additional matrix addition step. Particles, containing biomolecules up to 2,000 Daltons, were analyzed with this technique. In the third approach, bioaerosols were deposited on a stainless steel target and analyzed by matrix-free infrared LDI. Deposited particulate, consisting of peptides and proteins up to 6,000 Daltons, was analyzed with this method. On-line single particle analysis of bioaerosols was conducted in an aerosol mass spectrometer. Prior to entering the mass spectrometer, particles were coated with matrix by condensation. The coated particles were then irradiated with a continuous triggering laser and the resulting ions were analyzed in a time-of-flight mass spectrometer. Single particles with peptides up to 1200 Da were observed in real-time in this study.

MPACT Scores for Shelley N. Jackson

A = 0
C = 0
A+C = 0
T = 0
G = 0
W = 0
TD = 0
TA = 0
calculated 2008-03-22 17:27:59

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