Dissertation Information for Suzanne R.L. YoungNAME:
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SCHOOL: ADVISORS: COMMITTEE MEMBERS: MPACT Status: Incomplete - Not_Inspected Title: CXXC finger protein 1 is important for hematopoiesis Abstract: This dissertation characterizes the role of CXXC Finger Protein 1 (CFP-1) during hematopoietic differentiation. CFP-1, encoded by the CXXC1 gene, is a transcriptional activator that binds unmethylated CpG motifs and is ubiquitously expressed. Mice lacking CFP-1 exhibit an embryonic lethal phenotype (E3.5-E6). Murine embryonic stem cells lacking CFP-1 are viable but unable to differentiate and exhibit 60-80% decrease in cytosine methylation. The zebrafish homologue of CFP-1 is ∼70% similar to the mouse CFP-1 and is expressed in zebrafish embryos in all tissues. Approximately 80% of CFP-1 morpholino oligonucleotide ( zCXXC1 MO) treated embryos exhibited few circulating erythrocytes and abnormal yolk sac morphology at 48 hpf. More than half of the zCXXC1 MO treated zebrafish exhibited cardiac edema, and ∼14% were dead at 24 hpf. These phenotypes were rescued upon co-injection of the CFP-1 mRNA with the zCXXC1 MO. Embryos treated with a control MO exhibited normal yolk sac morphology and normal erythrocyte circulation. Genomic DNA from zCXXC1 MO treated embryos exhibited a 60% reduction of global cytosine methylation compared to DNA isolated from untreated or control MO treated embryos. |
MPACT Scores for Suzanne R.L. YoungA = 0 Advisors and Advisees Graph |