Login

Publications  •  Project Statistics

Glossary  •  Schools  •  Disciplines
People Search: 
   
Title/Abstract Search: 

Dissertation Information for Jingping Ge

NAME:
- Jingping Ge

DEGREE:
- Ph.D.

DISCIPLINE:
- Pathology and Laboratory Medicine

SCHOOL:
- Indiana University (USA) (2005)

ADVISORS:
- Richard L. Gregory

COMMITTEE MEMBERS:
- Margherita R. Fontana
- Thomas E. Davis
- Mark E. Lasbury

MPACT Status: Incomplete - Not_Inspected

Title: Identification of the surface-associated proteins and functions of enolase of Streptococcus mutans

Abstract: Streptococcus mutans is a gram positive bacterium, which can cause dental cavities and bacterial endocarditis. Surface-associated proteins may play an important role in bacterial adherence and cell metabolism in pathophysiological processes. In this study, the surface-associated proteins of S. mutans were separated by electrophoresis and identified by mass spectrometry. A total of 42 surface-associated proteins were identified, and the isoelectric points of those proteins were in the range of 3 to 10. The putative functions of S. mutans surface-associated proteins were adhesion factors, catalytic enzymes, transporters, and hypothetical proteins. Enolase, a critical enzyme in the glycolytic pathway, was confirmed as a surface-associated protein, and the interactions of enolase with human plasminogen and salivary mucins were demonstrated using transmission electron microscopy, ELISA, and Western blotting. These interactions may facilitate S. mutans attachment, clearance, or breach of the bloodstream barrier in bacterial endocarditis. The crucial amino acids for these binding activities of enolase to plasminogen and salivary mucins were studied by site-mutagenesis. It was demonstrated that the last two C-terminal lysine residues of enolase were crucial for plasminogen-binding activity, but the binding domain of enolase to salivary mucin II needs further study.

MPACT Scores for Jingping Ge

A = 0
C = 0
A+C = 0
T = 0
G = 0
W = 0
TD = 0
TA = 0
calculated 2010-10-14 13:48:39

Advisors and Advisees Graph