The file SoluPred.wk3.uue contains a uuencoded Excel spreadsheet saved in .wk3 format for Lotus on DOS machines. This spreadsheet allows one to predict the solubility of recombinant proteins in E. coli based on the amino acid content. A user types in the content of several amino acids and the spreadsheet predicts whether the protein will be soluble based on the method of Wilkinson and Harrison, as published in their paper "Predicting the solubility of recombinant proteins in Escherichia coli" Bio/Technology, vol. 9, p. 443-448 (1991). A few errors noted in this paper have been corrected, and the corrected model for protein solubility has been used in this spreadsheet. The spreadsheet was originally prepared by Dr. Harrison. The layout was slightly modified by Dan Diaz (BL275@cleveland.freenet.edu). Description: Lotus version of an Excel 4.0 spreadsheet to predict the solubility of recombinant proteins in E. coli