From bl275@kanga.INS.CWRU.Edu Tue Mar 21 17:42:10 1995
From: bl275@cleveland.Freenet.Edu (Dan Diaz)
To: archive@bio.indiana.edu
Subject: Lotus for DOS spreadsheet to predict protein solubility
Reply-To: bl275@cleveland.Freenet.Edu (Dan Diaz)
Content-Length: 4212
X-Lines: 77
Status: RO

The file SoluPred.wk3.uue contains a uuencoded Excel spreadsheet
saved in .wk3 format for Lotus on DOS machines.

This spreadsheet allows one to predict the solubility of
recombinant proteins in E. coli based on the amino acid
content.  A user types in the content of several amino acids
and the spreadsheet predicts whether the protein will be
soluble based on the method of Wilkinson and Harrison, as
published in their paper "Predicting the solubility of recombinant
proteins in Escherichia coli" Bio/Technology, vol. 9, p. 443-448
(1991).  A few errors noted in this paper have been corrected, and the
corrected model for  protein solubility has been used in this spreadsheet.

The spreadsheet was originally prepared by Dr. Harrison.  The layout was
slightly modified by Dan Diaz (BL275@cleveland.freenet.edu).

Description: Lotus version of an Excel 4.0 
             spreadsheet to predict the solubility 
             of recombinant proteins in E. coli


BEGIN-------cut here-------CUT HERE-------PART 01
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end
END-------cut here-------CUT HERE-------PART 01