PRIMERS This is a Macintosh program for designing primers for PCRȘ. Sequences can be entered by hand, from plain text files, from DNA Inspector files, or from IBI MacVector files. Up to 10 forward and 10 reverse primers can be compared. The program uses the method of Rychlik and Rhoads to calculate the thermodynamics of melting. Possible interfering secondary structures between primers and target are also shown. Instructions for use are given in the HELP section of the About Primers... menu item under the Apple Menu. The program is a SuperCard standalone project. It uses 1600K of memory, which is characteristic of these projects. The color looks reasonably good if your monitor is set to 256 colors. System 7 compatible. This is freeware. We make no claims for the efficacy of PRIMERS for any commercial or educational use. You may freely copy PRIMERS, but do not change the authorship or credits. The program has been packed as a Compact Pro self-extracing archive and BinHexed. Send bug reports to Greg Bristol at GREGB@lbes.medsch.ucla.edu.Dear IUBio Archives, Here is version 1.2 of Primers for Macintosh. It replaces version 1.1 which had a problem saving and reading files on Mac IIsi's. Primers evaluates oligonucleotides as possible PCR primers. The target sequence can be read in as text, MacVector, or DNA Inspector files. The file has been compressed with Compact Pro and binhexed. Any bugs or problems, please email Greg Bristol at the Lab of Molecular Medicine and Structural Biology (formerly LBES): gregb@lbes.medsch.ucla.edu