This spreadsheet allows one to predict the solubility of
recombinant proteins in E. coli based on the amino acid
content.  A user types in the content of several amino acids
and the spreadsheet predicts whether the protein will be
soluble based on the method of Wilkinson and Harrison, as
published in their paper "Predicting the solubility of recombinant
proteins in Escherichia coli" Bio/Technology, vol. 9, p. 443-448
(1991).  A few errors noted in this paper have been corrected, and the
corrected model for protein solubility has been used in this spreadsheet.

The spreadsheet is being emailed in two formats:
1. solupred.xl.hqx
An .HQX encoded version for Excel 4.0 for the Macintosh
2. solupred.wk3.uue
A uuencoded version for Lotus for DOS machines

The original spreadsheet was sent to me by Dr. Roger Harrison
of the University of Oklahoma, who asked my help in finding
the spreadsheet a home on the Internet.  I thought IUBio the
best place to post it.  I have made some slight modifications in
the interface, the rest remains Dr. Harrison's work.

Please feel free to contact me if I can supply any further
information.
Dan Diaz
BL275@cleveland.freenet.edu