LOCUS ECOLAC 7477 bp ds-DNA BCT 15-DEC-1988 DEFINITION E.coli lactose operon with lacI, lacZ, lacY and lacA genes. ACCESSION J01636 J01637 K01483 K01793 KEYWORDS acetyltransferase; beta-D-galactosidase; galactosidase; lac operon; lac repressor protein; lacA gene; lacI gene; lacY gene; lacZ gene; lactose permease; mutagenesis; palindrome; promoter; thiogalactoside acetyltransferase. SOURCE Escherichia coli DNA [1],[4],[5],[8],[10]; mRNA [2],[3],[6]; clone lambda-h80dlac DNA [7],[9]; clone puk217 [11]; pgm8 [12]. ORGANISM Escherichia coli Prokaryota; Bacteria; Gram-negative facultatively anaerobic rods; Enterobacteriaceae. REFERENCE 1 (bases 1243 to 1266) AUTHORS Gilbert,W. and Maxam,A. TITLE The nucleotide sequence of the lac operator JOURNAL Proc Nat Acad Sci USA 70, 3581-3584 (1973) STANDARD full staff_review REFERENCE 2 (bases 1246 to 1308) AUTHORS Maizels,N.M. TITLE The nucleotide sequence of the lactose messenger ribonucleic acid transcribed from the UV5 promoter mutant of Escherichia coli JOURNAL Proc Nat Acad Sci USA 70, 3585-3589 (1973) STANDARD full staff_review REFERENCE 3 (bases 1146 to 1282) AUTHORS Dickson,R.C., Abelson,J., Barnes,W.M. and Reznikoff,W.S. TITLE Genetic regulation: The lac control region JOURNAL Science 187, 27-35 (1975) STANDARD full staff_review REFERENCE 4 (bases 1227 to 1271) AUTHORS Gilbert,W., Maxam,A. and Mirzabekov,A. TITLE Contacts between the lac repressor and DNA revealed by methylation JOURNAL (in) Kjeldgaard,N.C. and Maaloe,O.(eds); Control of ribosome synthesis: 138-143; Academic Press, New York (1976) STANDARD full staff_review REFERENCE 5 (bases 1242 to 1268) AUTHORS Heyneker,H.L., Shine,J., Goodman,H.M., Boyer,H.W., Rosenberg,J., Dickerson,R.E., Narang,S.A., Itakura,K., Lin,S. and Riggs,A.D. TITLE Synthetic lac operator is functional in vivo JOURNAL Nature 263, 748-752 (1976) STANDARD full staff_review REFERENCE 6 (bases 51 to 264) AUTHORS Steege,D.A. TITLE 5'-terminal nucleotide sequence of Escherichia coli lactose repressor mRNA: Features of translational initiation and reinitiation sites JOURNAL Proc Nat Acad Sci USA 74, 4163-4167 (1977) STANDARD full staff_review REFERENCE 7 (bases 1 to 81) AUTHORS Calos,M.P. TITLE DNA sequence for a low-level promoter of the lac repressor and an "up" promoter mutation JOURNAL Nature 274, 762-765 (1978) STANDARD full staff_review REFERENCE 8 (bases 49 to 1161) AUTHORS Farabaugh,P.J. TITLE Sequence of the lacI gene JOURNAL Nature 274, 765-769 (1978) STANDARD full staff_review REFERENCE 9 (bases 4306 to 5804) AUTHORS Buechel,D.E., Gronenborn,B. and Mueller-Hill,B. TITLE Sequence of the lactose permease gene JOURNAL Nature 283, 541-545 (1980) STANDARD full staff_review REFERENCE 10 (bases 1183 to 1291) AUTHORS Weiher,H. and Schaller,H. TITLE Segment-specific mutagenesis: Extensive mutagenesis of a lac promoter/operator element JOURNAL Proc Nat Acad Sci USA 79, 1408-1412 (1982) STANDARD full staff_review REFERENCE 11 (bases 1287 to 4364) AUTHORS Kalnins,A., Otto,K., Ruether,U. and Mueller-Hill,B. TITLE Sequence of the lacZ gene of Escherichia coli JOURNAL EMBO J 2, 593-597 (1983) STANDARD full staff_review REFERENCE 12 (bases 5646 to 7477) AUTHORS Hediger,M.A., Johnson,D.F., Nierlich,D.P. and Zabin,I. TITLE DNA sequence of the lactose operon: The lacA gene and the transcriptional termination region JOURNAL Proc Nat Acad Sci USA 82, 6414-6418 (1985) STANDARD full staff_review REFERENCE 13 (sites; UV5 mRNA transcripts and operator mutants) AUTHORS Gilbert,W., Maizels,N. and Maxam,A. TITLE Sequences of controlling regions of the lactose operon JOURNAL Cold Spring Harb Symp Quant Biol 38, 845-855 (1974) STANDARD full staff_review REFERENCE 14 (sites; operator mutational analysis) AUTHORS Gilbert,W., Gralla,J., Majors,A.J. and Maxam,A. TITLE Lactose operator sequences and the action of lac repressor JOURNAL (in) Sund,H. and Blauer,G. (eds.); Protein-Ligand Interactions: 193-207; Walter de Gruyter, New York (1975) STANDARD full staff_review REFERENCE 15 (sites; S1 and mung bean nuclease action on operator DNA) AUTHORS Marians,K.J. and Wu,R. TITLE Structure of the lactose operator JOURNAL Nature 260, 360-363 (1976) STANDARD full staff_review REFERENCE 16 (sites; class I, II and III promoter mutant analysis) AUTHORS Dickson,R.C., Abelson,J., Johnson,P., Reznikoff,W.S. and Barnes,W.M. TITLE Nucleotide sequence changes produced by mutations in the lac promoter of Escherichia coli JOURNAL J Mol Biol 111, 65-75 (1977) STANDARD full staff_review REFERENCE 17 (sites; lacI mutant analysis) AUTHORS Miller,J.H., Coulondre,C. and Farabaugh,P.J. TITLE Correlation of nonsense sites in the lacI gene with specific codons in the nucleotide sequence JOURNAL Nature 274, 770-775 (1978) STANDARD full staff_review REFERENCE 18 (sites; Tn5, Tn9 and Tn10 insertion sites in lac region) AUTHORS Miller,J.H., Calos,M.P. and Galas,D.J. TITLE Genetic and sequencing studies of the specificity of transposition into the lac region of E. coli JOURNAL Cold Spring Harb Symp Quant Biol 45, 243-257 (1981) STANDARD full staff_review REFERENCE 19 (sites; lacI promoter mutation UJ177) AUTHORS Calos,M.P. and Miller,J.H. TITLE DNA sequence alteration resulting from a mutation impairing promoter function in the lac repressor gene JOURNAL Mol Gen Genet 178, 225-227 (1980) STANDARD full staff_review REFERENCE 20 (sites; palindromic dimer operator; see separate sequence entry under synthetic sequences) AUTHORS Betz,J.L. and Sadler,J.R. TITLE Variants of a cloned synthetic lactose operator: I. A palindromic dimer lactose operator derived from one strand of the cloned 40-base pair operator JOURNAL Gene 13, 1-12 (1981) STANDARD full staff_review REFERENCE 21 (sites; natural operator sequence) AUTHORS Sadler,J.R. and Tecklenburg,M. TITLE Cloning and characterization of the natural lactose operator JOURNAL Gene 13, 13-23 (1981) STANDARD full staff_review REFERENCE 22 (sites; operator mutational analysis) AUTHORS Betz,J.L. and Sadler,J.R. TITLE Variants of a cloned synthetic lactose operator: II. Chloramphenicol-resistant revertants retaining a lactose opeator in the CAT gene of plasmid pBR325 JOURNAL Gene 15, 187-200 (1981) STANDARD full staff_review REFERENCE 23 (sites; lacI-Q deletion) AUTHORS Calos,M.P. and Miller,J.H. TITLE The DNA sequence change resulting from the I-Q1 mutation, which greatly increases promoter strength JOURNAL Mol Gen Genet 183, 559-560 (1981) STANDARD full staff_review REFERENCE 24 (sites; RNA polymerase UV5 promoter interaction) AUTHORS Chenchick,A., Beabealashvilli,R. and Mirzabekov,A. TITLE Topography of interaction of Escherichia coli RNA polymerase subunits with lac UV5 promoter JOURNAL FEBS Lett 128, 46-50 (1981) STANDARD full staff_review REFERENCE 25 (sites; lacY mutational analysis) AUTHORS Mieschendahl,M., Buechel,D., Bocklage,H. and Mueller-Hill,B. TITLE Mutations in the lacY gene of Escherichia coli define functional organization of lactose permease JOURNAL Proc Nat Acad Sci USA 78, 7652-7656 (1981) STANDARD full staff_review REFERENCE 26 (sites; lacI-promoted transcription termination) AUTHORS Horowitz,H. and Platt,T. TITLE A termination site for lacI transcription is between the CAP site and the lac promoter JOURNAL J Biol Chem 257, 11740-11746 (1982) STANDARD full staff_review REFERENCE 27 (sites; wt and UV5 promoter sequence studies) AUTHORS Klein,R.D. and Wells,R.D. TITLE Effects of neighboring DNA homopolymers on the biochemical and physical properties of the Escherichia coli lactose promoter: I. Cloning and characterization studies JOURNAL J Biol Chem 257, 12954-12961 (1982) STANDARD full staff_review REFERENCE 28 (sites; UV5 promoter mutational analysis) AUTHORS Russell,D.R. and Bennett,G.N. TITLE Construction and analysis of in vivo activity of E. coli promoter hybrids and promoter mutants that alter the -35 to -10 spacing JOURNAL Gene 20, 231-243 (1982) STANDARD full staff_review REFERENCE 29 (sites; perfectly symmetric operator sequence) AUTHORS Sadler,J.R., Sasmor,H. and Betz,J.L. TITLE A perfectly symmetric lac operator binds the lac repressor very tightly JOURNAL Proc Nat Acad Sci USA 80, 6785-6789 (1983) STANDARD full staff_review REFERENCE 30 (sites; lacI mRNA termination site) AUTHORS Cone,K.C., Sellitti,M.A. and Steege,D.A. TITLE Lac repressor mRNA transcription terminates in vivo in the lac control region JOURNAL J Biol Chem 258, 11296-11304 (1983) STANDARD full staff_review REFERENCE 31 (sites; distamycin and actinomycin binding to promoter) AUTHORS Van Dyke,M.W. and Dervan,P.B. TITLE Footprinting with MPE-Fe(II). Complementary-strand analyses of distamycin- and actinomycin-binding sites on heterogeneous DNA JOURNAL Cold Spring Harb Symp Quant Biol 47, 347-353 (1983) STANDARD full staff_review REFERENCE 32 (sites; lacI deletion studies) AUTHORS Glickman,B.W. and Ripley,L.S. TITLE Structural intermediates of deletion mutagenesis: A role for palindromic DNA JOURNAL Proc Nat Acad Sci USA 81, 512-516 (1984) STANDARD full staff_review REFERENCE 33 (sites; promoter mutational studies) AUTHORS Mandecki,W., Goldman,R.A., Powell,B.S. and Caruthers,M.H. TITLE Lac up-promoter mutants with increased homology to the consensus promoter sequence JOURNAL J Bacteriol 164, 1353-1355 (1985) STANDARD full staff_review REFERENCE 34 (sites; DNAase I studies with promoter sequence) AUTHORS Straney,D.C. and Crothers,D.M. TITLE Intermediates in transcription initiation from the E. coli lac UV5 promoter JOURNAL Cell 43, 449-459 (1985) STANDARD full staff_review REFERENCE 35 (sites; ribosomal binding and translation initiation for lacZ) AUTHORS Looman,A.C., de Gruyter,M., Vogelaar,A. and van Knippenberg,P.H. TITLE Effects of heterologous ribosomal binding sites on the transcription and translation of the lacZ gene of Escherichia coli JOURNAL Gene 37, 145-154 (1985) STANDARD full staff_review REFERENCE 36 (sites; insertion sequence IS1 integration in lacZ; see separate sequence entry for the IS1T sequence) AUTHORS Malamy,M.H., Rahaim,P.T., Hoffman,C.S., Baghdoyan,D., O'Connor,M.B. and Miller,J.F. TITLE A frameshift mutation at the junction of an IS1 insertion within lacZ restores beta-galactosidase activity via formation of an active lacZ-IS1 fusion protein JOURNAL J Mol Biol 181, 551-555 (1985) STANDARD full staff_review REFERENCE 37 (sites; DNAase I studies with promoter) AUTHORS Spassky,A., Kirkegaard,K. and Buc,H. TITLE Changes in the DNA structure of the lac UV5 promoter during formation of an open complex with Escherichia coli RNA polymerase JOURNAL Biochemistry-USA 24, 2723-2731 (1985) STANDARD full staff_review COMMENT [1] first reports a 27 bp operator(sites 1240-1266) with two-fold symmetries; the operator has also been defined to be bases 1246-1266 or bases 1239-1273 [5]. [4] explores the ability of lac repressor protein to affect methylation of operator DNA. [5] argues that DNA on both sides of the 21 bp operator (bases 1246-1266) affects repressor binding but that the sequences of this DNA are probably not critical. [3] gives a larger sequence known as the promoter-operator region for the wild-type, whereas [2] and [10] give portions of this region for the mutant strain UV5. Within the promoter region, bases 1162-1199 are identified as the catabolite gene activator protein binding site (cap) and bases 1200-1245 are the RNA polymerase interaction site. [6] reports a sequence for the 5'end of the lacI (repressor) gene and discusses restart in mutant strains. [7] presents a sequence for the lacI promoter region and identifies an I-Q mutation which enhances lacI transcription approximately ten-fold. [8] gives a complete sequence for lacI which agrees with the known lac repressor sequence. [10] examines the promoter-operator region in the UV5 strain (lac109) and studies 23 mutant derivatives of this sequence. This sequence agrees with known protein sequences for the lacZ, lacY and lacA enzymes. [9] notes that the fMet codon is not present for lacA and suggests that the "ttg" codon (5727-5729), which immediately precedes the mature N-terminal asparagine codon, is the start codon. The cds for lacZ, lacY and lacA are included on a single mRNA transcript. FEATURES from to/span description pept 79 1161 lac repressor protein (lacI; gtg start codon) pept 1284 4358 beta-d-galactosidase (lacZ) pept 4410 5663 lactose permease (lacY) pept 5727 6338 thiogalactoside acetyltransferase (lacA; ttg start codon) mRNA 51 1230 lacI (repressor) mRNA; preferred in vivo 3' end [8],[30] mRNA 1246 > 4358 lacZ mRNA [2],[3] mut 16 16 c in wild-type; t in 'up' promoter mutant I-Q [7] refnumbr 51 51 numbered 1 in [7],[8]; zero not used[7] refnumbr 79 81 numbered codon 1 in [6] signal 1162 1199 cap protein binding site refnumbr 1162 1162 numbered 1 in [3]; zero not used variant 1183 1186 ttag in wild-type; aatt in strain UV5 [10] mut 1209 1211 gct in wild-type; gt in mutant l305 [3] mut 1212 1212 t in wild-type; a in mutant l241 [3] mut 1230 1230 c in wild-type; a in mutant p-r-1a [3] variant 1237 1238 gt in wild-type; aa in strain UV5 [10] mut 1242 1245 gtgg in wild-type; ttca in synthetic operator [5] refnumbr 1243 1243 sequence not numbered in [1] signal 1246 1266 lac repressor protein binding site refnumbr 1246 1246 numbered 1 in [2],[10]; zero not used refnumbr 1247 1247 numbered 1 in [5]; zero used revision 1262 1262 c in [2],[3],[4],[5],[10]; g in [1] mut 1267 1268 tc in wild-type; tg in synthetic operator [5] refnumbr 1281 1281 (c) numbered 1 on comp strand in [4] variant 1282 1291 ctatgaccat in wild-type; gatccggcca in strain UV5 [10] refnumbr 1287 1287 numbered 1 in [11] refnumbr 4305 4305 numbered 1 in [9] refnumbr 5646 5646 numbered 4401 in [12] BASE COUNT 1739 a 1991 c 2004 g 1743 t ORIGIN HindII site [7]; 7.9 min on K12 map. BACTERIA:ECOLAC Length: 7477 May 7, 1989 20:07 Check: 4810 .. 1 GACACCATCG AATGGCGCAA AACCTTTCGC GGTATGGCAT GATAGCGCCC 51 GGAAGAGAGT CAATTCAGGG TGGTGAATGT GAAACCAGTA ACGTTATACG 101 ATGTCGCAGA GTATGCCGGT GTCTCTTATC AGACCGTTTC CCGCGTGGTG 151 AACCAGGCCA GCCACGTTTC TGCGAAAACG CGGGAAAAAG TGGAAGCGGC 201 GATGGCGGAG CTGAATTACA TTCCCAACCG CGTGGCACAA CAACTGGCGG 251 GCAAACAGTC GTTGCTGATT GGCGTTGCCA CCTCCAGTCT GGCCCTGCAC 301 GCGCCGTCGC AAATTGTCGC GGCGATTAAA TCTCGCGCCG ATCAACTGGG 351 TGCCAGCGTG GTGGTGTCGA TGGTAGAACG AAGCGGCGTC GAAGCCTGTA 401 AAGCGGCGGT GCACAATCTT CTCGCGCAAC GCGTCAGTGG GCTGATCATT 451 AACTATCCGC TGGATGACCA GGATGCCATT GCTGTGGAAG CTGCCTGCAC 501 TAATGTTCCG GCGTTATTTC TTGATGTCTC TGACCAGACA CCCATCAACA 551 GTATTATTTT CTCCCATGAA GACGGTACGC GACTGGGCGT GGAGCATCTG 601 GTCGCATTGG GTCACCAGCA AATCGCGCTG TTAGCGGGCC CATTAAGTTC 651 TGTCTCGGCG CGTCTGCGTC TGGCTGGCTG GCATAAATAT CTCACTCGCA 701 ATCAAATTCA GCCGATAGCG GAACGGGAAG GCGACTGGAG TGCCATGTCC 751 GGTTTTCAAC AAACCATGCA AATGCTGAAT GAGGGCATCG TTCCCACTGC 801 GATGCTGGTT GCCAACGATC AGATGGCGCT GGGCGCAATG CGCGCCATTA 851 CCGAGTCCGG GCTGCGCGTT GGTGCGGATA TCTCGGTAGT GGGATACGAC 901 GATACCGAAG ACAGCTCATG TTATATCCCG CCGTCAACCA CCATCAAACA 951 GGATTTTCGC CTGCTGGGGC AAACCAGCGT GGACCGCTTG CTGCAACTCT 1001 CTCAGGGCCA GGCGGTGAAG GGCAATCAGC TGTTGCCCGT CTCACTGGTG 1051 AAAAGAAAAA CCACCCTGGC GCCCAATACG CAAACCGCCT CTCCCCGCGC 1101 GTTGGCCGAT TCATTAATGC AGCTGGCACG ACAGGTTTCC CGACTGGAAA 1151 GCGGGCAGTG AGCGCAACGC AATTAATGTG AGTTAGCTCA CTCATTAGGC 1201 ACCCCAGGCT TTACACTTTA TGCTTCCGGC TCGTATGTTG TGTGGAATTG 1251 TGAGCGGATA ACAATTTCAC ACAGGAAACA GCTATGACCA TGATTACGGA 1301 TTCACTGGCC GTCGTTTTAC AACGTCGTGA CTGGGAAAAC CCTGGCGTTA 1351 CCCAACTTAA TCGCCTTGCA GCACATCCCC CTTTCGCCAG CTGGCGTAAT 1401 AGCGAAGAGG CCCGCACCGA TCGCCCTTCC CAACAGTTGC GCAGCCTGAA 1451 TGGCGAATGG CGCTTTGCCT GGTTTCCGGC ACCAGAAGCG GTGCCGGAAA 1501 GCTGGCTGGA GTGCGATCTT CCTGAGGCCG ATACTGTCGT CGTCCCCTCA 1551 AACTGGCAGA TGCACGGTTA CGATGCGCCC ATCTACACCA ACGTAACCTA 1601 TCCCATTACG GTCAATCCGC CGTTTGTTCC CACGGAGAAT CCGACGGGTT 1651 GTTACTCGCT CACATTTAAT GTTGATGAAA GCTGGCTACA GGAAGGCCAG 1701 ACGCGAATTA TTTTTGATGG CGTTAACTCG GCGTTTCATC TGTGGTGCAA 1751 CGGGCGCTGG GTCGGTTACG GCCAGGACAG TCGTTTGCCG TCTGAATTTG 1801 ACCTGAGCGC ATTTTTACGC GCCGGAGAAA ACCGCCTCGC GGTGATGGTG 1851 CTGCGTTGGA GTGACGGCAG TTATCTGGAA GATCAGGATA TGTGGCGGAT 1901 GAGCGGCATT TTCCGTGACG TCTCGTTGCT GCATAAACCG ACTACACAAA 1951 TCAGCGATTT CCATGTTGCC ACTCGCTTTA ATGATGATTT CAGCCGCGCT 2001 GTACTGGAGG CTGAAGTTCA GATGTGCGGC GAGTTGCGTG ACTACCTACG 2051 GGTAACAGTT TCTTTATGGC AGGGTGAAAC GCAGGTCGCC AGCGGCACCG 2101 CGCCTTTCGG CGGTGAAATT ATCGATGAGC GTGGTGGTTA TGCCGATCGC 2151 GTCACACTAC GTCTGAACGT CGAAAACCCG AAACTGTGGA GCGCCGAAAT 2201 CCCGAATCTC TATCGTGCGG TGGTTGAACT GCACACCGCC GACGGCACGC 2251 TGATTGAAGC AGAAGCCTGC GATGTCGGTT TCCGCGAGGT GCGGATTGAA 2301 AATGGTCTGC TGCTGCTGAA CGGCAAGCCG TTGCTGATTC GAGGCGTTAA 2351 CCGTCACGAG CATCATCCTC TGCATGGTCA GGTCATGGAT GAGCAGACGA 2401 TGGTGCAGGA TATCCTGCTG ATGAAGCAGA ACAACTTTAA CGCCGTGCGC 2451 TGTTCGCATT ATCCGAACCA TCCGCTGTGG TACACGCTGT GCGACCGCTA 2501 CGGCCTGTAT GTGGTGGATG AAGCCAATAT TGAAACCCAC GGCATGGTGC 2551 CAATGAATCG TCTGACCGAT GATCCGCGCT GGCTACCGGC GATGAGCGAA 2601 CGCGTAACGC GAATGGTGCA GCGCGATCGT AATCACCCGA GTGTGATCAT 2651 CTGGTCGCTG GGGAATGAAT CAGGCCACGG CGCTAATCAC GACGCGCTGT 2701 ATCGCTGGAT CAAATCTGTC GATCCTTCCC GCCCGGTGCA GTATGAAGGC 2751 GGCGGAGCCG ACACCACGGC CACCGATATT ATTTGCCCGA TGTACGCGCG 2801 CGTGGATGAA GACCAGCCCT TCCCGGCTGT GCCGAAATGG TCCATCAAAA 2851 AATGGCTTTC GCTACCTGGA GAGACGCGCC CGCTGATCCT TTGCGAATAC 2901 GCCCACGCGA TGGGTAACAG TCTTGGCGGT TTCGCTAAAT ACTGGCAGGC 2951 GTTTCGTCAG TATCCCCGTT TACAGGGCGG CTTCGTCTGG GACTGGGTGG 3001 ATCAGTCGCT GATTAAATAT GATGAAAACG GCAACCCGTG GTCGGCTTAC 3051 GGCGGTGATT TTGGCGATAC GCCGAACGAT CGCCAGTTCT GTATGAACGG 3101 TCTGGTCTTT GCCGACCGCA CGCCGCATCC AGCGCTGACG GAAGCAAAAC 3151 ACCAGCAGCA GTTTTTCCAG TTCCGTTTAT CCGGGCAAAC CATCGAAGTG 3201 ACCAGCGAAT ACCTGTTCCG TCATAGCGAT AACGAGCTCC TGCACTGGAT 3251 GGTGGCGCTG GATGGTAAGC CGCTGGCAAG CGGTGAAGTG CCTCTGGATG 3301 TCGCTCCACA AGGTAAACAG TTGATTGAAC TGCCTGAACT ACCGCAGCCG 3351 GAGAGCGCCG GGCAACTCTG GCTCACAGTA CGCGTAGTGC AACCGAACGC 3401 GACCGCATGG TCAGAAGCCG GGCACATCAG CGCCTGGCAG CAGTGGCGTC 3451 TGGCGGAAAA CCTCAGTGTG ACGCTCCCCG CCGCGTCCCA CGCCATCCCG 3501 CATCTGACCA CCAGCGAAAT GGATTTTTGC ATCGAGCTGG GTAATAAGCG 3551 TTGGCAATTT AACCGCCAGT CAGGCTTTCT TTCACAGATG TGGATTGGCG 3601 ATAAAAAACA ACTGCTGACG CCGCTGCGCG ATCAGTTCAC CCGTGCACCG 3651 CTGGATAACG ACATTGGCGT AAGTGAAGCG ACCCGCATTG ACCCTAACGC 3701 CTGGGTCGAA CGCTGGAAGG CGGCGGGCCA TTACCAGGCC GAAGCAGCGT 3751 TGTTGCAGTG CACGGCAGAT ACACTTGCTG ATGCGGTGCT GATTACGACC 3801 GCTCACGCGT GGCAGCATCA GGGGAAAACC TTATTTATCA GCCGGAAAAC 3851 CTACCGGATT GATGGTAGTG GTCAAATGGC GATTACCGTT GATGTTGAAG 3901 TGGCGAGCGA TACACCGCAT CCGGCGCGGA TTGGCCTGAA CTGCCAGCTG 3951 GCGCAGGTAG CAGAGCGGGT AAACTGGCTC GGATTAGGGC CGCAAGAAAA 4001 CTATCCCGAC CGCCTTACTG CCGCCTGTTT TGACCGCTGG GATCTGCCAT 4051 TGTCAGACAT GTATACCCCG TACGTCTTCC CGAGCGAAAA CGGTCTGCGC 4101 TGCGGGACGC GCGAATTGAA TTATGGCCCA CACCAGTGGC GCGGCGACTT 4151 CCAGTTCAAC ATCAGCCGCT ACAGTCAACA GCAACTGATG GAAACCAGCC 4201 ATCGCCATCT GCTGCACGCG GAAGAAGGCA CATGGCTGAA TATCGACGGT 4251 TTCCATATGG GGATTGGTGG CGACGACTCC TGGAGCCCGT CAGTATCGGC 4301 GGAATTCCAG CTGAGCGCCG GTCGCTACCA TTACCAGTTG GTCTGGTGTC 4351 AAAAATAATA ATAACCGGGC AGGCCATGTC TGCCCGTATT TCGCGTAAGG 4401 AAATCCATTA TGTACTATTT AAAAAACACA AACTTTTGGA TGTTCGGTTT 4451 ATTCTTTTTC TTTTACTTTT TTATCATGGG AGCCTACTTC CCGTTTTTCC 4501 CGATTTGGCT ACATGACATC AACCATATCA GCAAAAGTGA TACGGGTATT 4551 ATTTTTGCCG CTATTTCTCT GTTCTCGCTA TTATTCCAAC CGCTGTTTGG 4601 TCTGCTTTCT GACAAACTCG GGCTGCGCAA ATACCTGCTG TGGATTATTA 4651 CCGGCATGTT AGTGATGTTT GCGCCGTTCT TTATTTTTAT CTTCGGGCCA 4701 CTGTTACAAT ACAACATTTT AGTAGGATCG ATTGTTGGTG GTATTTATCT 4751 AGGCTTTTGT TTTAACGCCG GTGCGCCAGC AGTAGAGGCA TTTATTGAGA 4801 AAGTCAGCCG TCGCAGTAAT TTCGAATTTG GTCGCGCGCG GATGTTTGGC 4851 TGTGTTGGCT GGGCGCTGTG TGCCTCGATT GTCGGCATCA TGTTCACCAT 4901 CAATAATCAG TTTGTTTTCT GGCTGGGCTC TGGCTGTGCA CTCATCCTCG 4951 CCGTTTTACT CTTTTTCGCC AAAACGGATG CGCCCTCTTC TGCCACGGTT 5001 GCCAATGCGG TAGGTGCCAA CCATTCGGCA TTTAGCCTTA AGCTGGCACT 5051 GGAACTGTTC AGACAGCCAA AACTGTGGTT TTTGTCACTG TATGTTATTG 5101 GCGTTTCCTG CACCTACGAT GTTTTTGACC AACAGTTTGC TAATTTCTTT 5151 ACTTCGTTCT TTGCTACCGG TGAACAGGGT ACGCGGGTAT TTGGCTACGT 5201 AACGACAATG GGCGAATTAC TTAACGCCTC GATTATGTTC TTTGCGCCAC 5251 TGATCATTAA TCGCATCGGT GGGAAAAACG CCCTGCTGCT GGCTGGCACT 5301 ATTATGTCTG TACGTATTAT TGGCTCATCG TTCGCCACCT CAGCGCTGGA 5351 AGTGGTTATT CTGAAAACGC TGCATATGTT TGAAGTACCG TTCCTGCTGG 5401 TGGGCTGCTT TAAATATATT ACCAGCCAGT TTGAAGTGCG TTTTTCAGCG 5451 ACGATTTATC TGGTCTGTTT CTGCTTCTTT AAGCAACTGG CGATGATTTT 5501 TATGTCTGTA CTGGCGGGCA ATATGTATGA AAGCATCGGT TTCCAGGGCG 5551 CTTATCTGGT GCTGGGTCTG GTGGCGCTGG GCTTCACCTT AATTTCCGTG 5601 TTCACGCTTA GCGGCCCCGG CCCGCTTTCC CTGCTGCGTC GTCAGGTGAA 5651 TGAAGTCGCT TAAGCAATCA ATGTCGGATG CGGCGCGACG CTTATCCGAC 5701 CAACATATCA TAACGGAGTG ATCGCATTGA ACATGCCAAT GACCGAAAGA 5751 ATAAGAGCAG GCAAGCTATT TACCGATATG TGCGAAGGCT TACCGGAAAA 5801 AAGACTTCGT GGGAAAACGT TAATGTATGA GTTTAATCAC TCGCATCCAT 5851 CAGAAGTTGA AAAAAGAGAA AGCCTGATTA AAGAAATGTT TGCCACGGTA 5901 GGGGAAAACG CCTGGGTAGA ACCGCCTGTC TATTTCTCTT ACGGTTCCAA 5951 CATCCATATA GGCCGCAATT TTTATGCAAA TTTCAATTTA ACCATTGTCG 6001 ATGACTACAC GGTAACAATC GGTGATAACG TACTGATTGC ACCCAACGTT 6051 ACTCTTTCCG TTACGGGACA CCCTGTACAC CATGAATTGA GAAAAAACGG 6101 CGAGATGTAC TCTTTTCCGA TAACGATTGG CAATAACGTC TGGATCGGAA 6151 GTCATGTGGT TATTAATCCA GGCGTCACCA TCGGGGATAA TTCTGTTATT 6201 GGCGCGGGTA GTATCGTCAC AAAAGACATT CCACCAAACG TCGTGGCGGC 6251 TGGCGTTCCT TGTCGGGTTA TTCGCGAAAT AAACGACCGG GATAAGCACT 6301 ATTATTTCAA AGATTATAAA GTTGAATCGT CAGTTTAAAT TATAAAAATT 6351 GCCTGATACG CTGCGCTTAT CAGGCCTACA AGTTCAGCGA TCTACATTAG 6401 CCGCATCCGG CATGAACAAA GCGCAGGAAC AAGCGTCGCA TCATGCCTCT 6451 TTGACCCACA GCTGCGGAAA ACGTACTGGT GCAAAACGCA GGGTTATGAT 6501 CATCAGCCCA ACGACGCACA GCGCATGAAA TGCCCAGTCC ATCAGGTAAT 6551 TGCCGCTGAT ACTACGCAGC ACGCCAGAAA ACCACGGGGC AAGCCCGGCG 6601 ATGATAAAAC CGATTCCCTG CATAAACGCC ACCAGCTTGC CAGCAATAGC 6651 CGGTTGCACA GAGTGATCGA GCGCCAGCAG CAAACAGAGC GGAAACGCGC 6701 CGCCCAGACC TAACCCACAC ACCATCGCCC ACAATACCGG CAATTGCATC 6751 GGCAGCCAGA TAAAGCCGCA GAACCCCACC AGTTGTAACA CCAGCGCCAG 6801 CATTAACAGT TTGCGCCGAT CCTGATGGCG AGCCATAGCA GGCATCAGCA 6851 AAGCTCCTGC GGCTTGCCCA AGCGTCATCA ATGCCAGTAA GGAACCGCTG 6901 TACTGCGCGC TGGCACCAAT CTCAATATAG AAAGCGGGTA ACCAGGCAAT 6951 CAGGCTGGCG TAACCGCCGT TAATCAGACC GAAGTAAACA CCCAGCGTCC 7001 ACGCGCGGGG AGTGAATACC ACGCGAACCG GAGTGGTTGT TGTCTTGTGG 7051 GAAGAGGCGA CCTCGCGGGC GCTTTGCCAC CACCAGGCAA AGAGCGCAAC 7101 AACGGCAGGC AGCGCCACCA GGCGAGTGTT TGATACCAGG TTTCGCTATG 7151 TTGAACTAAC CAGGGCGTTA TGGCGGCACC AAGCCCACCG CCGCCCATCA 7201 GAGCCGCGGA CCACAGCCCC ATCACCAGTG GCGTGCGCTG CTGAAACCGC 7251 CGTTTAATCA CCGAAGCATC ACCGCCTGAA TGATGCCGAT CCCCACCCCA 7301 CCAAGCAGTG CGCTGCTAAG CAGCAGCGCA CTTTGCGGGT AAAGCTCACG 7351 CATCAATGCA CCGACGGCAA TCAGCAACAG ACTGATGGCG ACACTGCGAC 7401 GTTCGCTGAC ATGCTGATGA AGCCAGCTTC CGGCCAGCGC CAGCCCGCCC 7451 ATGGTAACCA CCGGCAGAGC GGTCGAC