I'll send NSEQTOOL in a few seconds. It's a program by R. Schnobel for SUN 4 or SPARCstations described in CABIOS 7 (1991), 341-346 for simultaneous display of multiple sequence alignments and protein backbone structure. NSEQTOOL(1) USER COMMANDS NSEQTOOL(1) NAME nseqtool - is a colour browser for aligned sequences plus 3D view of a backbone. AVAILABILITY nseqtool runs under UNIX on a SUN-4 or SPARC workstation. It is made available by typing prepare_proteins. DESCRIPTION The display of a set of aligned sequences is combined with a stick representation of a folded protein backbone. The user may select one protein out of the alignment whose sequential and structural views will get intimately coupled. The interactive graphical environment provides for direct control of objects: Picking and highlighting residues, scrolling the sequence display, permuting the colour group- ing, rotation and scaling are performed with a mouse click. AUTHOR Raimund Schnobel and Arthur Lesk 1989, 1990. EXAMPLE Here is a sample session with nseqtool. Suntools (Open a shell window) CR /home/schnobel/projects/align/files/cd4 BRK 2rhe ATTACH 1 QUIT (This will read the alignment from a file supplied by the user and the coordinates from BROOKHAVEN file 2RHE.BRK. The stick display is attached to the first sequence.) (Note that the upper-case commands should be typed in the command field near the bottom of the screen. Click the left mouse button to refresh the picture.) INPUT FILE The coordinate file is in BROOKHAVEN format. The sequence file format looks like this: 2RHE ESVLTQP-PSASGTPGQRVTISCTGSATDIGS----NSVIWYQQVPGKA PKLLIYYNDLLPSGVSDRFSASKSGTSASLAISGLESEDEADYYCAA WND-SLDEPGFGGGTKLTVLGQPK* KOL VL ESVLTQP-PSASGTPGQRVTISCTGTSSNIGS----ITVNWYQQLPGMA PKLLIYRDAMRPSGVPTRFSGSKSGTSASLAISGLEAEDESDYYCAS WNS-SDNSYVFGTGTKVTVLGQPKANPTVT LFPPSSEELQANKATLVCLI* NEW VL -SVLTQP-PSVSGAPGQRVTISCTGSSSNIGA---GNHVKWYQQLPGTA PKLLIF-------HNNARFSVSKSGSSATLAITGLQAEDEADYYCQS YDR---SLRVFGGGTKLTVLRQPKAAPSVTLFPPSSEELQANKAT* RELATED PROGRAMS The program Cameleon by G.M. Morris, Oxford, has a rich set of features, but handles only two proteins, and has no structure display. RESTRICTIONS The upper limit of the number of sequences is set to 1000 each having at most 2000 residues. No side chains can be displayed. DEVICES REQUIRED nseqtool requires a colour graphics screen with a mouse. It is implemented on SUN-4 and SPARC workstations under Sun- tools.