[compost_tea] PARASITISM OF TRICHODERMA ON ROOT-KNOT NEMATODES

From: Tom Jaszewski <tom_at_livesoil.com>
Date: Wed, 27 Oct 2004 18:57:31 -0700

I like trichoderma more and more..

 

 

190. PARASITISM OF TRICHODERMA ON ROOT-KNOT NEMATODES - MECHANISMS AND
IMPROVED

BIOCONTROL

E. Sharon1, I. Chet2 and Y. Spiegel1

1Division of Nematology , ARO, Volcani Center, P.O.B. 6, Bet-Dagan 50250,
Israel

2Department of Biological Chemistry, Weizmann Institute of Science, Israel

vpshedna_at_volcani.agri.gov.il

The fungus Trichoderma harzianum (asperellum) exhibits biocontrol activity
against the root-knot nematode

Meloidogyne javanica. Direct fungal parasitism is one of various possible
mechanisms by which the fungus can act

against the nematode juveniles (J2) and eggs. The fungal hyphae coil around
the J2 and penetrate them, as well as

to nematode eggs. A constitutive GFP (green fluorescent protein)
-transformant was used to observe the fungal

parasitism on nematode eggs and juveniles, using laser scanning confocal
microscopy. The involvement of

proteolytic and chitinilytic enzymes in the process was demonstrated using
inductive GFP-fungal transformants.

The protease and chitinase genes turned on during the interaction between
the fungus and the nematodes. The

direct fungal parasitism process on the nematode in vitro could be improved
using antibodies. Antibodies against

root-knot nematodes, that label the surface of M. javanica J2 and eggs,
bound also to the fungal spores and

agglutinated them. The presence of these antibodies enhanced the attachment
of the spores to the nematodes.

This resulted in a significant enhancement of fungal parasitism on the
nematodes. Moreover, the binding of

antibodies to the spores enhanced their germination and thus improved the
fungal parasitism on the nematodes.

 








Received on Wed Oct 27 2004 - 22:27:43 EDT

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