[compost_tea] Re: Cornelious vessels

From: dkemnitz2000 <dkemnitz2000_at_yahoo.com>
Date: Fri, 18 Mar 2005 01:00:09 -0000

So Ron how do you put them back into lag phase or to sleep(whichever
is necessary to store them) in general. Can you slowly raise the CO2
level, remove there nutrients, add an excess of nutrients(like sugar
or nitrogen),etc.  Dennis




--- In compost_tea_at_yahoogroups.com, "rhc1050" <RHC1050@h...> wrote:
>
> --- In compost_tea_at_yahoogroups.com, "Jack Hickman"
<jackhickman_at_b...>
> wrote:
> >
> > I have a question.I had one of these vessels.These are used to
> > dispense Coke and Pepsi at soda fountains.I used welder's oxygen
> which
> > is pure oxygen.I set the vessel at 60 psi and pumped the oxygen
in
> the
> > vessel.Let set for 1 hour with,with pressure up.This gave me
> anywhere
> > from 40 to 60 ppm dissolved oxygen in it.
> > My question, would this make a better ACT?
>
> You are getting very close to the way one would grow large scale 
> monocultures of bacteria or cells under laboratory conditions,
where
> the culture is not nutrient limited and maximum cell density is
> desired.  However, at high concentrations, oxygen can be toxic.
> Typically, we would fill our reactor with media, bubble air at a
rate
> that was determined to be as fast as possible without shearing the
> cells, measure oxygen levels and set our meter such that the
measured
> level was 100%.  We would then add bacteria or cells and run at an
> oxygen level somewhat less than 100%, typically 70%.   At some
point,
> the culture density would be such that we could no longer maintain
> the desired level with air and we would switch to oxygen.  We also
> monitored pH and adjusted for optimum and fed selected nutrients
late
> in the culture cycle.
>
> To my mind, this is exactly how I would like to brew ACT, at least
> for experimental purposes (it wouldn't surprise me if Elaine
already
> has).  I'm working with an electrical engineer friend in NZ to see
> just how cheap we could build a set of automated controls.  He's a
> pretty amazing guy, it wouldn't surprise me if he came up with
> something practical.  The two flies in the ointment will be the
cost
> of a decent oxygen probe and how the powers that be finally rule
on
> feeding molasses (though I am making sum produce an organic medium
> without the use of added sugars).
>
> ron




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Received on Thu Mar 17 2005 - 22:04:49 EST

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