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gene shuffling to create herbicide resistant crops



The patent application below shows an approach that may be new to many. What is
being done is the genes for herbicide tolerance or genes that can be converted
to herbicide tolerance are scrambled then screened for herbicide tolerance
activity before being inserted into the crop. Are crops with such scrambled
genes substantially equivalent to un-modified crops? I expect that the
technique will appeal to the shuffled brains of the regulators at EPA, USDA and
FDA and that CFIA will be happy to agree to anything the boss wants.

Title: US20020058249A1: DNA SHUFFLING TO PRODUCE HERBICIDE SELECTIVE CROPS 2002

SUBRAMANIAN, VENKITESWARAN; SAN DIEGO, CA, United States of America
STEMMER, WILLEM P.C.; LOS GATOS, CA, United States of America
CASTLE, LINDA A.; MOUNTAIN VIEW, CA, United States of America
MUCHHAL, UMESH S.; MOUNTAIN VIEW, CA, United States of America
SIEHL, DANIEL L.; MENLO PARK, CA, United States of America


Methods of shuffling DNA to obtain recombinant herbicide tolerance nucleic
acids encoding proteins having new or improved herbicide tolerance activities,
libraries of shuffled herbicide tolerance nucleic acids, transgenic plants and
DNA shuffling mixtures are provided.

What is claimed is:
1. A method of obtaining a recombinant herbicide tolerance nucleic acid which
can confer tolerance to an herbicide upon a plant in which the recombinant
herbicide tolerance nucleic acid is present, the method comprising: (i)
recombining a plurality of variant forms of one or more parental nucleic acid,
wherein the plurality of variant forms comprises segments derived from the
parental nucleic acid, wherein the parental nucleic acid encodes an herbicide
tolerance activity, or can be shuffled to encode an herbicide tolerance
activity, and wherein the plurality of variant forms differ from each other in
at least one nucleotide, to produce a library of recombinant nucleic acids;
(ii) screening the library to identify at least one recombinant herbicide
tolerance nucleic acid, wherein the recombinant herbicide tolerance nucleic
acid encodes an activity which confers herbicide tolerance to a cell.

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