Re: [compost_tea] Re: Cornelious vessels

From: Jack Hickman <jackhickman_at_bellsouth.net>
Date: Fri, 18 Mar 2005 07:52:28 -0600

Hi Robert
  If 02 is not needed in the ACT.Then would contest stirring make ACT?
  ----- Original Message -----
  From: Robert Norsen
  To: compost_tea_at_yahoogroups.com
  Sent: Friday, March 18, 2005 1:55 AM
  Subject: Re: [compost_tea] Re: Cornelious vessels


  Hi Leo and Jack

  Are you thinking that DO is something that goes into water and stays ther=
e?. That once loaded with O2 that that amount of O2 is significant. Like =
you could charge waer with O2, drink it and it would supply your body or t=
he race horse with O2. Nope.

  O2 is in the water all right but it is the O2 that is tranported minute b=
y minute by bubbles ( or waves) to the ACT that provide the significant O2 =
 
  Air breathing animals can't get significant O2 by drinking it. Microbes =
and fish breath water to get O2 from water. Frogs get O2 thru their hair.=
 ( Grin) . Yes you need to aerate with lots of air passing thru the water=
. This bubble action must also circulate the water which it can do very wl=
l.
   Leo Fernandez <helamanf_at_yahoo.com> wrote:

    Hi

    How much O2 need in my ATC....6ppm and I have aerobic
    tea......but how I can stay in this range...easy.....I
    need "x" vol. of tea in agitation....why....the O2 if
    no have agitation cross in the water and not
    dissolved..

    .i.e.
    in the single microbe fermentation .... when I put
    azotobacter in my fermenter I need 275 rpm whit 65% of
    saturation of O2 to make cyst of azotobacter in
    48hr...


    but if I have wrong plase tell me....


    Thx

    Leo From Chihuahua


    --- Jack Hickman wrote:
> This is all new for me.I was a millwright for a
> long,long time.I guess that is why I like tinkering
> with things.
> I used the oxygen water for my race horse(I don't
> race horses anymore)The problem I had,the oxygen
> would not stay in the water a significant time.
> ----- Original Message -----
> From: rhc1050
> To: compost_tea_at_yahoogroups.com
> Sent: Thursday, March 17, 2005 5:41 PM
> Subject: [compost_tea] Re: Cornelious vessels
>
>
>
> --- In compost_tea_at_yahoogroups.com, "Jack Hickman"
>
> wrote:
> >
> > I have a question.I had one of these
> vessels.These are used to
> > dispense Coke and Pepsi at soda fountains.I used
> welder's oxygen
> which
> > is pure oxygen.I set the vessel at 60 psi and
> pumped the oxygen in
> the
> > vessel.Let set for 1 hour with,with pressure
> up.This gave me
> anywhere
> > from 40 to 60 ppm dissolved oxygen in it.
> > My question, would this make a better ACT?
>
> You are getting very close to the way one would
> grow large scale
> monocultures of bacteria or cells under laboratory
> conditions, where
> the culture is not nutrient limited and maximum
> cell density is
> desired. However, at high concentrations, oxygen
> can be toxic.
> Typically, we would fill our reactor with media,
> bubble air at a rate
> that was determined to be as fast as possible
> without shearing the
> cells, measure oxygen levels and set our meter
> such that the measured
> level was 100%. We would then add bacteria or
> cells and run at an
> oxygen level somewhat less than 100%, typically
> 70%. At some point,
> the culture density would be such that we could no
> longer maintain
> the desired level with air and we would switch to
> oxygen. We also
> monitored pH and adjusted for optimum and fed
> selected nutrients late
> in the culture cycle.
>
> To my mind, this is exactly how I would like to
> brew ACT, at least
> for experimental purposes (it wouldn't surprise me
> if Elaine already
> has). I'm working with an electrical engineer
> friend in NZ to see
> just how cheap we could build a set of automated
> controls. He's a
> pretty amazing guy, it wouldn't surprise me if he
> came up with
> something practical. The two flies in the
> ointment will be the cost
> of a decent oxygen probe and how the powers that
> be finally rule on
> feeding molasses (though I am making sum produce
> an organic medium
> without the use of added sugars).
>
> ron
>
>
>
>
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Received on Fri Mar 18 2005 - 09:43:53 EST

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